Table 1S.  Protein identifications for microaerobically and anaerobically grown H. influenzae Rd strain KW20.

ORF Position Coding Strand Protein Length (aa) Protein ID COG ID Protein Description Grown Cells Data
Start Stop microaerobically anaerobically
2 1021 + 339 HI0001       COG0057 glyceraldehyde-3-phosphate dehydrogenase (gapdH) + +
1190 3013 + 607 HI0002       COG1022 long chain fatty acid coA ligase, putative - +/-
3050 3838 - 262 HI0003       COG0561 conserved hypothetical protein +/- -
3854 4318 - 154 HI0004       COG0319 conserved hypothetical protein +/- -
4579 5391 - 270 HI0005       COG1526 fdhD protein (fdhD) - -
5662 8748 + 1028 HI0006m      COG0243 Formate dehydrogenase large subunit + +/-
8750 9688 + 312 HI0007       COG0437 formate dehydrogenase, beta subunit (fdxH) +/- -
9681 10397 + 238 HI0008       COG2864 formate dehydrogenase, gamma subunit (fdxI) - +
10467 11375 + 302 HI0009       COG3058 fdhE protein (fdhE) +/- +/-
11414 11854 - 146 HI0010       COG0456 ribosomal-protein-alanine acetyltransferase (rimI) +/- -
11857 12261 - 134 HI0011       COG3050 DNA polymerase III, psi subunit (holD) - +/-
12367 13359 + 330 HI0012       COG2813 conserved hypothetical protein - -
13423 14331 - 302 HI0013       COG1159 GTP-binding protein (era) - -
14328 15011 - 227 HI0014       COG0571 ribonuclease III (rnc) +/- -
15013 16062 - 349 HI0015       COG0681 signal peptidase I (lepB) + +
16071 17867 - 598 HI0016       COG0481 GTP-binding membrane protein (lepA) +/- +/-
18035 18418 - 127 HI0017       COG3445 conserved hypothetical protein + +
18676 19335 + 219 HI0018       COG0692 uracil DNA glycosylase (ung) - -
19405 20829 - 474 HI0019       COG0621 conserved hypothetical protein +/- -
21248 22687 - 479 HI0020       COG0471 transport protein, putative +/- +/-
22689 23939 - 416 HI0021       COG1767/COG3697 citG protein (citG) +/- -
24267 25769 - 500 HI0022       COG3051 citrate lyase, alpha chain (citF) + +
25784 26659 - 291 HI0023       COG2301 citrate lyase, beta chain (citE) + +
26656 26943 - 95 HI0024       COG3052 citrate lyase, gamma chain (citD) + +/-
26981 27988 - 335 HI0025       COG3053 citrate lyase ligase (citC) - -
28239 29201 - 320 HI0026       COG0320 lipoate biosynthesis protein A (lipA) +/- +/-
29255 29893 - 212 HI0027       COG0321 lipoate biosynthesis protein B (lipB) - -
29895 30173 - 92 HI0028       COG2921 conserved hypothetical protein +/- -
30229 31410 - 393 HI0029       COG1686 penicillin-binding protein 5 (dacA) - +/-
31426 32289 - 287 HI0030       COG0797 lipoprotein, putative +/- -
32341 33456 - 371 HI0031       COG0772 rod shape-determining protein (rodA) +/- +/-
33446 35401 - 651 HI0032       COG0768 penicillin-binding protein 2 (pbp2) +/- +/-
35422 35889 - 155 HI0033       COG1576 conserved hypothetical protein +/- -
35943 36251 - 102 HI0034       COG0799 conserved hypothetical protein - -
36376 38031 - 551 HI0035       COG2985 conserved hypothetical protein +/- +/-
38237 40015 + 592 HI0036       COG1133 ABC transporter, ATP-binding protein + +/-
40064 41200 + 378 HI0037       COG1077 rod shape-determining protein (mreB) +/- -
41280 42335 + 351 HI0038       COG1792 rod shape-determining protein (mreC) +/- +/-
42335 42823 + 162 HI0039       COG2891 rod shape-determining protein (mreD) - -
42874 43650 - 258 HI0040          -    conserved hypothetical protein - +/-
43660 44463 - 267 HI0041       COG0708 exodeoxyribonuclease III (xthA) +/- +/-
44511 45185 - 224 HI0042       COG0564 conserved hypothetical protein - +/-
45185 46249 - 354 HI0043       COG3768 conserved hypothetical transmembrane protein +/- -
46309 46971 - 220 HI0044       COG0670 conserved hypothetical transmembrane protein +/- +/-
47298 47852 - 184 HI0045       COG3054    conserved hypothetical protein +/- +/-
47919 48281 + 120 HI0046       COG2824 alkylphosphonate uptake protein (phnA) + +/-
48334 48972 - 212 HI0047       COG0800 4-hydroxy-2-oxoglutarate aldolase/2-deydro-3-deoxyphosphogluconate aldolase (eda) + +/-
48991 49848 - 285 HI0048       COG1028 oxidoreductase + -
49848 50792 - 314 HI0049       COG0524 2-dehydro-3-deoxygluconokinase (kdgK) - +/-
50812 52086 - 424 HI0050m      COG1593 conserved hypothetical transmembrane protein - +/-
52092 52589 - 165 HI0051       COG3090 conserved hypothetical transmembrane protein - -
52628 53614 - 328 HI0052       COG1638 conserved hypothetical protein +/- -
53641 54669 - 342 HI0053       COG1063 zinc-type alcohol dehydrogenase +/- +/-
54779 55579 + 266 HI0054       COG2186 uxu operon regulator (uxuR) +/- -
55607 56791 + 394 HI0055       COG1312 mannonate dehydratase (uxuA) +/- +/-
56844 57557 - 237 HI0056       COG0861 conserved hypothetical transmembrane protein + -
57655 59484 - 609 HI0057       COG0322 excinuclease ABC, subunit C (uvrC) +/- +/-
59486 60250 - 254 HI0058       COG1212 3-deoxy-D-manno-octulosonate cytidylyltransferase (kdsB) - -
60321 61319 - 332 HI0059       COG1663 conserved hypothetical protein +/- -
61392 63155 - 587 HI0060       COG1132 ABC transporter, ATP-binding protein (msbA) - +/-
63196 64206 - 788 HI0061       COG2333/COG0658 recombination protein (rec2) +/- +/-
65821 66258 + 145 HI0062       COG1734 dnaK suppressor protein (dksA) - -
66506 67864 + 452 HI0063       COG0617 poly(A) polymerase (pcnB) +/- -
67873 68355 + 160 HI0064       COG0801 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine-pyrophosphokinase (folK) +/- -
68431 68907 + 158 HI0065       COG0802 conserved hypothetical protein +/- -
68915 70213 + 246 HI0066       COG0860/COG1388 N-acetylmuramoyl-L-alanine amidase, putative +/- +/-
70214 72103 + 629 HI0067       COG0323 DNA mismatch repair protein (mutL) - +/-
72111 73046 + 311 HI0068       COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase (trpX) +/- -
73052 75997 + 981 HI0069       COG1391 glutamate-ammonia-ligase adenylyltransferase (glnE) +/- +/-
76082 77758 - 558 HI0070       COG0497 DNA repair protein (recN) +/- +/-
77902 78687 - 261 HI0072       COG0061 conserved hypothetical protein +/- -
78751 79455 + 234 HI0071       COG0576 heat shock protein (grpE) + +
79468 79812 - 114 HI0073       COG1708 H. influenzae predicted coding region HI0073 - -
79805 80245 - 146 HI0074          -    H. influenzae predicted coding region HI0074 - -
80526 82649 + 707 HI0075       COG1328 anaerobic ribonucleoside-triphosphate reductase (nrdD) +/- +/-
82767 83627 + 286 HI0076       COG1946 acyl-CoA thioesterase II (tesB) +/- -
83638 84504 + 288 HI0077       COG2833 H. influenzae predicted coding region HI0077 - -
84580 85959 - 459 HI0078       COG0215 cysteinyl-tRNA synthetase (cysS) + -
86062 86571 + 169 HI0079       COG0652 peptidyl-prolyl cis-trans isomerase B (ppiB) + -
86575 87006 + 143 HI0080       COG3692 conserved hypothetical protein +/- -
87148 87936 + 262 HI0081       COG0084 conserved hypothetical protein - +/-
88193 88459 + 88 HI0082          -    H. influenzae predicted coding region HI0082 - +/-
88474 88689 + 71 HI0083          -    H. influenzae predicted coding region HI0083 - -
88739 89062 - 107 HI0084       COG0526 thioredoxin (trxM) + +
89182 90177 - 331 HI0085       COG1052 D-lactate dehydrogenase, fermentative (ldhA) + -
90190 91335 - 381 HI0086       COG0626 cystathionine gamma-synthase (metB) +/- +
92148 93425 - 425 HI0087       COG0498 threonine synthase (thrC) + +
93468 94412 - 314 HI0088       COG0083 homoserine kinase (thrB) +/- -
94425 96872 - 815 HI0089       COG0460/COG0527 aspartokinase I / homoserine dehydrogenase I (thrA) +/- +/-
97196 97909 + 237 HI0090       COG0325 conserved hypothetical protein - +/-
97949 99085 - 378 HI0091       COG1929 conserved hypothetical protein +/- -
99094 100353 - 419 HI0092       COG2610 H. influenzae predicted coding region HI0092 +/- +/-
100475 101581 - 368 HI0093       COG2508 conserved hypothetical protein - -
101783 102103 - 106 HI0094       COG0451 H. influenzae predicted coding region HI0094 - -
102194 102364 + 56 HI0094a      COG1349 Uncharacterized protein - -
102453 103208 - 251 HI0095       COG0500 conserved hypothetical protein - -
103542 104117 + 191 HI0096          -    H. influenzae predicted coding region HI0096 - -
104390 105274 + 294 HI0097       COG1840 iron(III) ABC transporter, periplasmic-binding protein (hitA) + +
105491 106912 + 473 HI0098       COG1178 iron(III) ABC transporter, permease protein (hitB) +/- -
106914 107969 + 351 HI0099       COG1130 iron(III) ABC transporter, ATP-binding protein (hitC) +/- -
108004 108399 - 131 HI0100       COG1876 H. influenzae predicted coding region HI0100 - -
108243 108683 - 146 HI0101       COG1876 H. influenzae predicted coding region HI0101 +/- +/-
108685 109818 - 377 HI0102       COG0624 succinyl-diaminopimelate desuccinylase (dapE) +/- +
109846 110190 - 114 HI0103       COG1393 conserved hypothetical protein - +/-
110269 112164 - 631 HI0104       COG0326 heat shock protein (htpG) + +
112381 113220 + 279 HI0105       COG0327 conserved hypothetical protein + -
113277 114665 - 462 HI0106       COG0541 signal recognition particle protein (ffh) +/- +/-
114997 116208 + 403 HI0107       COG1253 conserved hypothetical protein - +/-
116337 117230 + 297 HI0108       COG2966/COG3610 conserved hypothetical protein +/- -
117319 117660 - 113 HI0109       COG3765 H. influenzae predicted coding region HI0109 +/- -
117827 119116 + 429 HI0110       COG0172 seryl-tRNA synthetase (serS) + +
119451 120080 + 209 HI0111       COG0625 glutathione transferase (bphH) + +
120092 120388 - 98 HI0112       COG2963 H. influenzae predicted coding region HI0112 +/- -
120441 122675 + 744 HI0113       COG1629 hemin receptor (hemR) - -
129317 131453 - 736 HIN1253+2    COG1092/COG0116 Predicted SAM-dependent methyltransferases +/- +/-
132027 132959 + 310 HIN1254      COG2821 Membrane-bound lytic murein transglycosylase +/- +/-
132944 133729 + 261 HI0118       COG1179 conserved hypothetical protein +/- +
133907 134920 + 337 HI0119       COG0803 conserved hypothetical protein + +
134970 135476 - 168 HI0120          -    H. influenzae predicted coding region HI0120 - -
135589 136950 - 453 HI0121       COG0773 conserved hypothetical protein + -
137335 138525 - 396 HI0122       COG0626 cystathionine beta-lyase (metC) - -
138800 139357 + 185 HI0123       COG0558 phosphatidylglycerophosphate synthase (pgsA) - -
139924 140454 - 176 HI0124       COG0221 inorganic pyrophosphatase (ppa) + +
140689 142005 + 438 HI0125       COG2252 conserved hypothetical protein +/- +/-
142163 143149 - 328 HI0126       COG1130 ferric ABC transporter, ATP-binding protein (afuC) + +
143193 145091 - 632 HI0129       COG1178 H. influenzae predicted coding region HI0129 - +/-
145395 146435 - 346 HI0131       COG1840 afuA protein + +
146601 147242 + 213 HI0132       COG0572 uridine kinase (udk) +/- +/-
147251 147838 + 195 HI0133       COG0717 deoxycytidine triphosphate deaminase (dcd) +/- -
147842 149017 + 391 HI0134          -    H. influenzae predicted coding region HI0134 +/- -
149017 150207 + 396 HI0135       COG2814 conserved hypothetical transmembrane protein +/- +/-
150287 151801 - 504 HI0136       COG1160 GTP-binding protein +/- +/-
152492 153262 - 256 HI0137       COG0847 DNA polymerase III, epsilon subunit (dnaQ) - +/-
153323 153787 + 154 HI0138       COG0328 ribonuclease H (rnh) - -
154039 155118 + 359 HI0139       COG3203 outer membrane protein P2 (ompP2) + +
155307 156452 - 381 HI0140       COG1820 N-acetylglucosamine-6-phosphate deacetylase (nagA) + -
156725 157537 - 270 HI0141       COG0363 glucosamine-6-phosphate isomerase (nagB) + +/-
157823 158704 - 293 HI0142       COG0329 N-acetylneuraminate lyase (nanA) + +
158715 159581 - 288 HI0143       COG1737 conserved hypothetical protein +/- -
159574 160476 - 300 HI0144       COG1940 glucose kinase, putative +/- +/-
160522 161208 - 228 HI0145       COG3010 conserved hypothetical protein +/- +/-
161558 162547 + 329 HI0146       COG1638 conserved hypothetical protein + +
162558 164459 + 633 HI0147       COG3090/COG1593 conserved hypothetical transmembrane protein +/- +/-
164623 165762 + 379 HI0148       COG3055 conserved hypothetical protein + +
165924 166130 + 68 HI0148.1        -    H. influenzae predicted coding region HI0148.1 +/- +/-
166166 166357 + 63 HI0149       COG2020 H. influenzae predicted coding region HI0149 - -
166400 167287 - 295 HI0150       COG0330 hflC protein (hflC) + -
167287 168519 - 410 HI0151       COG0330 hflK protein (hflK) + +
168629 169336 - 235 HI0152       COG2091 H. influenzae predicted coding region HI0152 +/- -
169396 170726 - 443 HIN1287      COG2704    Anaerobic C4-dicarboxylate transporter +/- +/-
170930 171160 - 76 HI0154       COG0236 acyl carrier protein (acpP) + +
171416